Detection and analysis of proteins modified by O-linked N-acetylglucosamine

Curr Protoc Mol Biol. 2002 Feb;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s57.

Abstract

The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

MeSH terms

  • Acetylglucosamine / analogs & derivatives
  • Acetylglucosamine / analysis*
  • Animals
  • Blotting, Western / methods
  • Carbohydrate Conformation
  • Cells, Cultured / chemistry
  • Chromatography, Affinity / methods
  • Galactosyltransferases
  • Glycoproteins / analysis*
  • Glycoproteins / chemistry
  • Glycoproteins / immunology
  • Glycosylation
  • Indicators and Reagents
  • Mammals / metabolism
  • Mass Spectrometry / methods
  • N-Acetylglucosaminyltransferases / analysis
  • Protein Processing, Post-Translational
  • Wheat Germ Agglutinins

Substances

  • Glycoproteins
  • Indicators and Reagents
  • N-acetylglucosaminylserine
  • N-acetylglucosaminylthreonine
  • Wheat Germ Agglutinins
  • succinylated wheat germ agglutinin
  • Galactosyltransferases
  • N-Acetylglucosaminyltransferases
  • O-GlcNAc transferase
  • Acetylglucosamine