Abstract
The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.
MeSH terms
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Acetylglucosamine / analogs & derivatives
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Acetylglucosamine / analysis*
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Animals
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Blotting, Western / methods
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Carbohydrate Conformation
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Cells, Cultured / chemistry
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Chromatography, Affinity / methods
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Galactosyltransferases
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Glycoproteins / analysis*
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Glycoproteins / chemistry
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Glycoproteins / immunology
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Glycosylation
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Indicators and Reagents
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Mammals / metabolism
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Mass Spectrometry / methods
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N-Acetylglucosaminyltransferases / analysis
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Protein Processing, Post-Translational
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Wheat Germ Agglutinins
Substances
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Glycoproteins
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Indicators and Reagents
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N-acetylglucosaminylserine
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N-acetylglucosaminylthreonine
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Wheat Germ Agglutinins
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succinylated wheat germ agglutinin
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Galactosyltransferases
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N-Acetylglucosaminyltransferases
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O-GlcNAc transferase
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Acetylglucosamine