The objective of this study was to identify the prevalence of symptoms of anorexia nervosa (EAT+) and dissatisfaction with body image among female adolescents in Florianópolis, Santa Catarina State, Brazil. The proportional sample (1,148 adolescents) was stratified according to position of schools in the socio-geographic regions and public/private school classification, and was subsequently distributed according to primary and secondary schools. EAT+ was identified through the Eating Attitudes Test and evaluation of body image through the Body Shape Questionnaire. Prevalence rates for EAT+ and dissatisfaction with body image were 15.6% and 18.8%, respectively. EAT+ was associated with age (10-13y) (OR = 1.542; p = 0.046); overweight/obesity (OR = 2.075; p < 0.001); dissatisfaction with body image (OR = 14.392; p < 0.001); and public schools (OR = 1.423; p = 0.041). Multiple logistic regression showed dissatisfaction with body image as the strongest independent risk factor for symptoms of anorexia nervosa (OR = 16.7; p < 0.001). Adolescents in Florianópolis show EAT+ rates similar to those observed in other regions of Brazil.