Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow

J Leukoc Biol. 2008 Jun;83(6):1459-66. doi: 10.1189/jlb.1207831. Epub 2008 Mar 27.


Galectin-1 (Gal-1) is a beta-galactoside-binding protein endowed with anti-inflammatory properties. The purpose of this study was to investigate the effects of endogenous and exogenous Gal-1 on neutrophil recruitment onto TNF-treated endothelium. The effect of human recombinant (hr)Gal-1 on markers of neutrophil activation (CD11b expression, P-selectin glycoprotein ligand 1, and L-selectin shedding) was also assessed. Gal-1 inhibited the platelet-activating factor-induced increase in CD11b expression in a concentration-dependent manner, as assessed by flow cytometry. To determine the effects of Gal-1 on neutrophil recruitment, an in vitro flow chamber was used: Preincubation of neutrophils with hrGal-1 significantly decreased the extent of capture, rolling, and adhesion on activated endothelial monolayers. This inhibition was shared with the endogenous protein, as knockdown of endothelial Gal-1 using small interfering RNA resulted in a significant increase in the number of cells captured and rolling. To verify the effects of Gal-1 in an in vivo system, intravital microscopy of Gal-1 null mice and their wild-type counterparts was performed. Leukocyte adhesion and emigration were increased significantly in the cremasteric circulation of Gal-1 null mice inflamed with IL-1beta. These findings indicate that Gal-1 functions to limit neutrophil recruitment onto a TNF-treated endothelium, a property that may underline its inhibitory effects in acute inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD11b Antigen / analysis
  • Cell Communication / drug effects
  • Cell Movement
  • Female
  • Galectin 1 / analysis
  • Galectin 1 / physiology*
  • Male
  • Membrane Glycoproteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Neutrophil Infiltration*
  • Platelet Activating Factor / antagonists & inhibitors
  • RNA, Small Interfering / pharmacology


  • CD11b Antigen
  • Galectin 1
  • Membrane Glycoproteins
  • P-selectin ligand protein
  • Platelet Activating Factor
  • RNA, Small Interfering