The effects of reactive oxygen species on calcium- and carbachol-induced contractile responses in beta-escin permeabilized rat bladder

Naunyn Schmiedebergs Arch Pharmacol. 2008 Dec;378(6):645-53. doi: 10.1007/s00210-008-0326-3. Epub 2008 Jul 19.


The effect of reactive oxygen species on contractions in beta-escin permeabilized rat detrusor was investigated. Cumulative calcium contractions were inhibited by hydrogen peroxide (H(2)O(2)) and hydroxyl (*OH) but not by superoxide (O(2) *). The sarcoplasmic reticulum calcium-ATPase inhibitor cyclopiazonic acid (CPA) and the mitochondrial blocker carbonyl cyanide p-trifluromethoxyphenylhydrazone (FCCP) decreased the calcium contractions, however in their presence, H(2)O(2) and *OH did not have further effect. Carbachol contractions were inhibited by either H(2)O(2)/*OH/O(2) * or CPA/FCCP. In the presence of CPA, carbachol contractions were not affected by H(2)O(2) and *OH but further decreased by O(2) *. On the other hand, only H(2)O(2) and *OH elicited additional inhibition in carbachol responses in the presence of FCCP. Inositol triphosphate contraction was inhibited by *OH whereas none of the radicals affect carbachol induced calcium sensitization. These results show that H(2)O(2) and *OH affects sarcoplasmic reticulum where O(2) * acts on mitochondria to change contractions in rat detrusor smooth muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium-Transporting ATPases / antagonists & inhibitors
  • Calcium-Transporting ATPases / physiology
  • Carbachol / pharmacology*
  • Contractile Proteins / physiology
  • Escin / pharmacology*
  • In Vitro Techniques
  • Indoles / pharmacology
  • Inositol 1,4,5-Trisphosphate Receptors / physiology
  • Male
  • Mitochondrial Proteins / physiology
  • Muscle Contraction / drug effects
  • Muscle Contraction / physiology
  • Muscle, Smooth / drug effects*
  • Muscle, Smooth / physiology
  • Permeability
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism*
  • Sarcoplasmic Reticulum / drug effects
  • Sarcoplasmic Reticulum / metabolism
  • Urinary Bladder / drug effects*
  • Urinary Bladder / physiology


  • Contractile Proteins
  • Indoles
  • Inositol 1,4,5-Trisphosphate Receptors
  • Mitochondrial Proteins
  • Reactive Oxygen Species
  • Escin
  • Carbachol
  • Calcium-Transporting ATPases
  • Calcium
  • cyclopiazonic acid