Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats

Phytother Res. 2008 Nov;22(11):1511-9. doi: 10.1002/ptr.2523.


To evaluate the effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats. Adult Wistar rats of either sex were used (n = 30). Colitis was induced by a single intracolonic administration of TNBS dissolved in 35% ethanol. The rats (n = 30) were divided into five groups (n = 6) and were treated with vehicle (ethanol), TNBS, Manuka honey (5 g/kg, p.o.), Manuka honey (10 g/kg, p.o.) or sulfasalazine (360 mg/kg, p.o.) body weight for 14 days. After completion of treatment, the animals were killed and the following parameters were assessed: morphological score, histological score and different antioxidant parameters.Manuka honey at different doses provided protection against TNBS-induced colonic damage. There was significant protection with Manuka honey 5 g/kg as well as with 10 g/kg body weight compared with the control (p < 0.001). All the treated groups showed reduced colonic inflammation and all the biochemical parameters were significantly reduced compared with the control in the Manuka honey treated groups (p < 0.001). Manuka honey at different doses restored lipid peroxidation as well as improved antioxidant parameters. Morphological and histological scores were significantly reduced in the low dose Manuka honey treated group (p < 0.001). In the inflammatory model of colitis, oral administration of Manuka honey 5 g/kg and Manuka honey 10 g/kg body weight significantly reduced the colonic inflammation. The present study indicates that Manuka honey is efficacious in the TNBS-induced rat colitis model, but these results require further confirmation in human studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Antioxidants / metabolism
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / prevention & control
  • Colon / drug effects*
  • Colon / metabolism
  • Colon / pathology
  • Disease Models, Animal
  • Glutathione / metabolism
  • Honey*
  • Inflammation / drug therapy
  • Inflammatory Bowel Diseases / chemically induced
  • Inflammatory Bowel Diseases / drug therapy*
  • Inflammatory Bowel Diseases / prevention & control
  • Lipid Peroxidation
  • Male
  • Peroxidase / metabolism
  • Rats
  • Rats, Wistar
  • Sulfasalazine / pharmacology
  • Superoxide Dismutase / metabolism
  • Trinitrobenzenesulfonic Acid / toxicity


  • Antioxidants
  • Sulfasalazine
  • Trinitrobenzenesulfonic Acid
  • Peroxidase
  • Superoxide Dismutase
  • Glutathione