A continuous wavelet transform algorithm for peak detection

Electrophoresis. 2008 Nov;29(20):4215-25. doi: 10.1002/elps.200800096.

Abstract

Contactless conductivity detector technology has unique advantages for microfluidic applications. However, the low S/N and varying baseline makes the signal analysis difficult. In this paper, a continuous wavelet transform-based peak detection algorithm was developed for CE signals from microfluidic chips. The Ridger peak detection algorithm is based on the MassSpecWavelet algorithm by Du et al. [Bioinformatics 2006, 22, 2059-2065], and performs a continuous wavelet transform on data, using a wavelet proportional to the first derivative of a Gaussian function. It forms sequences of local maxima and minima in the continuous wavelet transform, before pairing sequences of maxima to minima to define peaks. The peak detection algorithm was tested against the Cromwell, MassSpecWavelet, and Linear Matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometer Peak Indication and Classification algorithms using experimental data. Its sensitivity to false discovery rate curve is superior to other techniques tested.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Artificial Intelligence
  • Electrophoresis, Capillary / methods
  • Microfluidic Analytical Techniques / methods*
  • Sensitivity and Specificity