Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice

Biochem Biophys Res Commun. 2008 Dec 26;377(4):1195-8. doi: 10.1016/j.bbrc.2008.10.156. Epub 2008 Nov 6.


Oxidative stress is implicated in atherogenesis; however most clinical trials with dietary antioxidants failed to show marked success in preventing atherosclerotic diseases. We have found that hydrogen (dihydrogen; H(2)) acts as an effective antioxidant to reduce oxidative stress [I. Ohsawa, M. Ishikawa, K. Takahashi, M. Watanabe, K. Nishimaki, K. Yamagata, K. Katsura, Y. Katayama, S, Asoh, S. Ohta, Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals, Nat. Med. 13 (2007) 688-694]. Here, we investigated whether drinking H(2)-dissolved water at a saturated level (H(2)-water) ad libitum prevents arteriosclerosis using an apolipoprotein E knockout mouse (apoE(-/-)), a model of the spontaneous development of atherosclerosis. ApoE(-/-) mice drank H(2)-water ad libitum from 2 to 6 month old throughout the whole period. Atherosclerotic lesions were significantly reduced by ad libitum drinking of H(2)-water (p=0.0069) as judged by Oil-Red-O staining series of sections of aorta. The oxidative stress level of aorta was decreased. Accumulation of macrophages in atherosclerotic lesions was confirmed. Thus, consumption of H(2)-dissolved water has the potential to prevent arteriosclerosis.

MeSH terms

  • Animals
  • Antioxidants / administration & dosage*
  • Antioxidants / chemistry
  • Aorta / pathology
  • Apolipoproteins E / genetics
  • Atherosclerosis / genetics
  • Atherosclerosis / pathology
  • Atherosclerosis / prevention & control*
  • Disease Models, Animal
  • Drinking
  • Hydrogen / administration & dosage*
  • Hydrogen / chemistry
  • Mice
  • Mice, Knockout
  • Oxidative Stress
  • Water / administration & dosage*
  • Water / chemistry


  • Antioxidants
  • Apolipoproteins E
  • Water
  • Hydrogen