Peptidase substrates via global peptide profiling

Nat Chem Biol. 2009 Jan;5(1):23-5. doi: 10.1038/nchembio.126. Epub 2008 Nov 16.


Peptide metabolism is a complex process that involves many proteins working in concert. Mass spectrometry-based global peptide profiling of mice lacking dipeptidyl peptidase 4 (DPP4) identified endogenous DPP4 substrates and revealed an unrecognized pathway during proline peptide catabolism that interlinks aminopeptidase and DPP4 activities. Together, these studies elucidate specific aspects of DPP4-regulated metabolism and, more generally, highlight the utility of global peptide profiling for studying peptide metabolism in vivo.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Dipeptidyl Peptidase 4 / chemistry
  • Dipeptidyl Peptidase 4 / genetics
  • Dipeptidyl Peptidase 4 / metabolism*
  • Dipeptidyl-Peptidase IV Inhibitors
  • Gene Expression Profiling*
  • Gene Expression Regulation / physiology*
  • Mice
  • Mice, Knockout
  • Protein Folding


  • Dipeptidyl-Peptidase IV Inhibitors
  • Dipeptidyl Peptidase 4

Associated data

  • PubChem-Substance/56364534