Enzyme catalyzed site-specific protein labeling and cell imaging with quantum dots

Chem Commun (Camb). 2008 Dec 7;(45):5927-9. doi: 10.1039/b812162a. Epub 2008 Oct 8.

Abstract

We have developed an efficient method for one-step covalent labeling of cell surface proteins with quantum dots based on enzyme catalyzed site-specific modification of short peptide tags.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Membrane / chemistry
  • Cells, Cultured
  • Coenzyme A / chemical synthesis
  • Coenzyme A / chemistry
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Microscopy, Fluorescence
  • Peptides / chemistry
  • Quantum Dots*
  • Transfection

Substances

  • Fluorescent Dyes
  • Membrane Proteins
  • Peptides
  • Coenzyme A