Enzyme catalyzed site-specific protein labeling and cell imaging with quantum dots

Murat Sunbul; Michelle Yen; Yekui Zou; Jun Yin

doi:10.1039/b812162a

Enzyme catalyzed site-specific protein labeling and cell imaging with quantum dots

Chem Commun (Camb). 2008 Dec 7:(45):5927-9. doi: 10.1039/b812162a. Epub 2008 Oct 8.

Authors

Murat Sunbul¹, Michelle Yen, Yekui Zou, Jun Yin

Affiliation

¹ Department of Chemistry, The University of Chicago, 929 East 57th Street, GCIS E505A, Chicago, IL 60637, USA.

PMID: 19030541
DOI: 10.1039/b812162a

Abstract

We have developed an efficient method for one-step covalent labeling of cell surface proteins with quantum dots based on enzyme catalyzed site-specific modification of short peptide tags.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Cell Membrane / chemistry
Cells, Cultured
Coenzyme A / chemical synthesis
Coenzyme A / chemistry
Fluorescent Dyes / chemistry
HeLa Cells
Humans
Membrane Proteins / chemistry*
Membrane Proteins / metabolism
Microscopy, Fluorescence
Peptides / chemistry
Quantum Dots*
Transfection

Substances

Fluorescent Dyes
Membrane Proteins
Peptides
Coenzyme A