The commercially important marine bivalve Mytilus galloprovincialis (Mediterranean mussel) is considered a valuable bioindicator, due to its exposure to various pollutants and extreme environmental conditions. Environmental responsive genes, such as the hsp90s, protect the structure and function of cells and accomplish a significant task in cellular homeostasis. To study the hsp90s in M. galloprovincialis a genomic library was screened and two hsp90s were isolated. Sequence analysis revealed that the two genes exhibit great similarities in both the 5' non-coding and the coding region but differ in the 3' non-coding region, as well as in three introns, due to the presence of repeated sequences. Few synonymous substitutions in the coding region of the genes result to an identical predicted polypeptide, which belongs to the cytoplasmic HSP90 subfamily. The 5' non-coding region contains a non-translated exon and multiple binding sites for various transcription factors. The presence of a p53 binding site in the promoter of the isolated genes raises questions about the possible implication of hsp90s in the molluscan leukemia.