Haplotype diversity in human mitochondrial DNA hypervariable regions I-III in the city of Caracas (Venezuela)

Forensic Sci Int Genet. 2008 Sep;2(4):e61-4. doi: 10.1016/j.fsigen.2007.12.009. Epub 2008 Feb 14.

Abstract

In order to expand the database of variable DNA for forensic identification purposes in Venezuela, we analyzed the sequence polymorphisms of mitochondrial DNA (mtDNA) hypervariable regions (HVR) I-III from 100 unrelated individuals from the city of Caracas, using PCR amplification and fluorescent-based capillary electrophoresis sequencing method. Dominant haplogroups corresponded to Native Americans followed by African ones. The inclusion of HVR III although useful for sub-haplogroup assignation, added little to the discrimination capacity of our database.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Black People / genetics
  • Complementarity Determining Regions / genetics*
  • DNA Primers
  • DNA, Mitochondrial / genetics*
  • DNA, Mitochondrial / isolation & purification
  • Electrophoresis, Capillary
  • Forensic Genetics / methods
  • Genetic Variation*
  • Haplotypes / genetics
  • Humans
  • Indians, North American / genetics
  • Polymerase Chain Reaction
  • Urban Population
  • Venezuela

Substances

  • Complementarity Determining Regions
  • DNA Primers
  • DNA, Mitochondrial