Involvement of PU.1 in mouse adar-1 gene transcription induced by high-dose esiRNA

Int J Biol Macromol. 2009 Aug 1;45(2):157-62. doi: 10.1016/j.ijbiomac.2009.04.021. Epub 2009 May 5.


Adar-1 gene plays an important role in the negative regulation of RNA interference. We previously showed that increased adar-1 mRNA level was associated with the rebound of gene expression after RNAi suppression. In this study, we identified a PU.1 binding site upstream from transcription start point of adar-1 gene and is essential for the promoter activity. Knockdown and over-expression of the PU.1 gene resulted in decreased and increased activity of adar-1 promoter, respectively. Our results suggest that transcription factor PU.1, could bind to the adar-1 promoter and play a key role in activating transcription of gene induced by high-dose esiRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / genetics*
  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Endoribonucleases / genetics*
  • Gene Expression Regulation
  • Gene Knockdown Techniques
  • Genes, Reporter / genetics
  • Mice
  • Promoter Regions, Genetic / genetics
  • Proto-Oncogene Proteins / metabolism*
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism
  • RNA-Binding Proteins
  • Trans-Activators / metabolism*
  • Transcription, Genetic*


  • Proto-Oncogene Proteins
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • Trans-Activators
  • proto-oncogene protein Spi-1
  • Endoribonucleases
  • ADARB1 protein, human
  • Adenosine Deaminase