Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4

J Immunol. 1990 Feb 1;144(3):902-8.


In the present study, we investigated the lymphokine production patterns in a series of CD4+ and CD8+ host-reactive T cell clones isolated from PBL of a SCID patient, who was immunologically reconstituted by two allogeneic fetal liver and thymus transplantations 13 years ago. We demonstrate that these donor-derived T cell clones, specifically reacting with the MHC Ag expressed on the recipient cells, do not produce IL-4 and do not express IL-4 mRNA upon Ag or polyclonal stimulations. In contrast, CD4+ tetanus toxin-specific T cell clones isolated from the same patient and having the same HLA phenotype produced normal amounts of IL-4 upon activation. These data suggest that the failure to produce IL-4 is a specific characteristic of these host-reactive clones and is not due to a genetic defect of the transplanted cells. Furthermore, different modes of activation resulted in simultaneous production of IL-5, IL-2, IFN-gamma, granulocyte/macrophage-CSF, and transcription of the TNF-beta gene by the host-reactive clones, indicating that the lack of IL-4 production is not related to the mode of activation. The finding that some of these clones produce significant levels of IL-5 but no IL-4 indicates that the IL-4 and IL-5 genes are not always coexpressed in activated human T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • Blotting, Northern
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8 Antigens
  • Chimera
  • Clone Cells
  • Colony-Stimulating Factors / biosynthesis
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Growth Substances / biosynthesis
  • Humans
  • Immunologic Deficiency Syndromes / immunology
  • Interferon-gamma / biosynthesis
  • Interleukin-2 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Interleukin-5 / biosynthesis
  • Lymphokines / biosynthesis*
  • Lymphokines / genetics
  • RNA, Messenger / genetics
  • Tetanus Toxoid / immunology
  • Transcription, Genetic


  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD8 Antigens
  • Colony-Stimulating Factors
  • Growth Substances
  • Interleukin-2
  • Interleukin-5
  • Lymphokines
  • RNA, Messenger
  • Tetanus Toxoid
  • Interleukin-4
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor