Characterization and antitumor effect of a novel polysaccharide from Grifola frondosa

J Agric Food Chem. 2009 Nov 11;57(21):10143-9. doi: 10.1021/jf9021338.


A novel polysaccharide, MZF, with a molecular mass of 23 kDa was isolated from Grifola frondosa . Results from methylation and (1)H NMR led to the conclusion that MZF is a heteropolysaccharide consisting of -->6)-alpha-D-Galp-(1--> (36.2%), -->3)-alpha-L-Fucp-(1--> (14.5%), -->6)-alpha-D-Manp-(1--> (9.4%), -->3)-beta-D-Glcp-(1--> (10.1%), alpha-D-Manp-(1--> (23.2%), and -->3,6)-beta-D-Glcp-(1--> (6.5%). Although MZF did not affect the proliferation of colon-26 cells in vitro, it significantly inhibited tumor growth in BALB/cA mice inoculated with colon-26 cancer cells. Moreover, MZF significantly induced the proliferation of splenocytes and peritoneal macrophages. The mRNA expression of IL-12p40, IL-2 and IFN-gamma were increased significantly in MZF-treated spleen. Furthermore, MZF augmented the percentage of IFN-gamma-producing cells in both splenic CD4(+) and CD8(+) T cells and tumor infiltrating CD4(+) and CD8(+) T cells and enhanced the cytotoxic activity of NK cells and CTLs. These results indicate that MZF is a novel effective immunomodulator that has antitumor activity associated with induced cell-mediated immunity.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Female
  • Grifola / chemistry*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Neoplasms / drug therapy
  • Neoplasms / immunology
  • Neoplasms / physiopathology
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Specific Pathogen-Free Organisms
  • Xenograft Model Antitumor Assays


  • Antineoplastic Agents
  • Polysaccharides