Multicenter analysis of novel and established variables associated with successful human islet isolation outcomes

Am J Transplant. 2010 Mar;10(3):646-56. doi: 10.1111/j.1600-6143.2009.02962.x. Epub 2010 Jan 5.

Abstract

Islet transplantation is a promising therapy used to achieve glycometabolic control in a select subgroup of individuals with type I diabetes. However, features that characterize human islet isolation success prior to transplantation are not standardized and lack validation. We conducted a retrospective analysis of 806 isolation records from 14 pancreas-processing laboratories, considering variables from relevant studies in the last 15 years. The outcome was defined as post-purification islet equivalent count, dichotomized into yields > or =315 000 or < or =220 000. Univariate analysis showed that donor cause of death and use of hormonal medications negatively influenced outcome. Conversely, pancreata from heavier donors and those containing elevated levels of surface fat positively influence outcome, as did heavier pancreata and donors with normal amylase levels. Multivariable logistic regression analysis identified the positive impact on outcome of surgically intact pancreata and donors with normal liver function, and confirmed that younger donors, increased body mass index, shorter cold ischemia times, no administration of fluid/electrolyte medications, absence of organ edema, use of University of Wisconsin preservation solution and a fatty pancreas improves outcome. In conclusion, this multicenter analysis highlights the importance of carefully reviewing all donor, pancreas and processing parameters prior to isolation and transplantation.

Publication types

  • Multicenter Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine / pharmacology
  • Adult
  • Aged
  • Allopurinol / pharmacology
  • Body Mass Index
  • Cold Ischemia
  • Female
  • Glutathione / pharmacology
  • Humans
  • Insulin / pharmacology
  • Islets of Langerhans / cytology*
  • Islets of Langerhans Transplantation / methods*
  • Male
  • Middle Aged
  • Organ Preservation Solutions / pharmacology
  • Pancreas / pathology
  • Raffinose / pharmacology
  • Regression Analysis
  • Retrospective Studies
  • Treatment Outcome

Substances

  • Insulin
  • Organ Preservation Solutions
  • University of Wisconsin-lactobionate solution
  • Allopurinol
  • Glutathione
  • Adenosine
  • Raffinose

Grant support