In situ detection and genotyping of individual mRNA molecules

Nat Methods. 2010 May;7(5):395-7. doi: 10.1038/nmeth.1448. Epub 2010 Apr 11.


Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / biosynthesis
  • Actins / genetics
  • Animals
  • Cell Line, Tumor
  • Cells, Cultured
  • Humans
  • Mice
  • Nucleic Acid Amplification Techniques / methods*
  • Point Mutation
  • RNA, Messenger / genetics*
  • Receptor, ErbB-2 / biosynthesis
  • Transcription, Genetic


  • Actins
  • RNA, Messenger
  • ERBB2 protein, human
  • Receptor, ErbB-2