Crystallization and preliminary X-ray diffraction analysis of West Nile virus

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt 5):558-62. doi: 10.1107/S1744309110009899. Epub 2010 Apr 30.


West Nile virus, a human pathogen, is closely related to other medically important flaviviruses of global impact such as dengue virus. The infectious virus was purified from cell culture using polyethylene glycol (PEG) precipitation and density-gradient centrifugation. Thin amorphously shaped crystals of the lipid-enveloped virus were grown in quartz capillaries equilibrated by vapor diffusion. Crystal diffraction extended at best to a resolution of about 25 A using synchrotron radiation. A preliminary analysis of the diffraction images indicated that the crystals had unit-cell parameters a approximately b approximately 480 A, gamma = 120 degrees , suggesting a tight hexagonal packing of one virus particle per unit cell.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cryoelectron Microscopy
  • Crystallization
  • Crystallography, X-Ray
  • West Nile virus / chemistry*
  • West Nile virus / isolation & purification
  • West Nile virus / ultrastructure