Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors

J Cell Biol. 2010 Jul 26;190(2):177-85. doi: 10.1083/jcb.201001035. Epub 2010 Jul 19.

Abstract

The nearly ubiquitous presence of repetitive centromere DNA sequences across eukaryotic species is in paradoxical contrast to their apparent functional dispensability. Centromeric chromatin is spatially delineated into the kinetochore-forming array of centromere protein A (CENP-A)-containing nucleosomes and the inner centromeric heterochromatin that lacks CENP-A but recruits the aurora B kinase that is necessary for correcting erroneous attachments to the mitotic spindle. We found that the self-perpetuating network of CENPs at the foundation of the kinetochore is intact at a human neocentromere lacking repetitive alpha-satellite DNA. However, aurora B is inappropriately silenced as a consequence of the altered geometry of the neocentromere, thereby compromising the error correction mechanism. This suggests a model wherein the neocentromere represents a primordial inheritance locus that requires subsequent generation of a robust inner centromere compartment to enhance fidelity of chromosome transmission.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aurora Kinase B
  • Aurora Kinases
  • Autoantigens / metabolism
  • Cells, Cultured
  • Centromere / genetics*
  • Centromere / metabolism
  • Centromere Protein A
  • Chromatin / genetics
  • Chromatin / metabolism
  • Chromosomal Proteins, Non-Histone / metabolism
  • Chromosomes, Human / genetics
  • Chromosomes, Human / metabolism
  • Chromosomes, Human / ultrastructure
  • Epigenesis, Genetic*
  • Fibroblasts / cytology
  • Fibroblasts / physiology
  • Humans
  • Mitosis / physiology*
  • Protein Serine-Threonine Kinases / metabolism*
  • Spindle Apparatus / metabolism

Substances

  • Autoantigens
  • CENPA protein, human
  • Centromere Protein A
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein Serine-Threonine Kinases