A combination of 670 nm and 810 nm diode lasers for wound healing acceleration in diabetic rats

Photomed Laser Surg. 2010 Oct;28(5):621-7. doi: 10.1089/pho.2009.2634.


Objectives: To study the effects of the combination of 670 nm and 810 nm diode lasers on diabetic wound healing parameters in rats.

Background: An alternative to traditional treatment modalities for diabetic ulcers is low-level laser therapy (LLLT). A number of published studies demonstrate the beneficial effects of LLLT, although several other studies also exist which indicate results to the contrary.

Methods: Four groups were present in our study: Diabetic-laser (n = 5), Diabetic-control (n = 4), Nondiabetic-laser (n = 5) and Nondiabetic-control (n = 5) groups. Two intervention (laser) groups underwent low level laser therapy using 670 nm diode laser (500 mW, 10 J, 48 s) in the wound context, and 810 nm diode laser (250 mW, 12 J, 50 s) to the wound margins. The wound area was measured using computer software after digital microscopic photography on days 0, 3, 6, 9, 12, 15, 20, and 24.

Results: There were no statistically significant differences between the diabetic and non-diabetic groups in the wound area, percentage of open wound area, and wound healing rate throughout the repeated measurements of the study. After seven days of low level laser therapy in the non-diabetic group, urine excretion was significantly increased in comparison with the control group.

Conclusion: Overall, our study showed results of measured wound healing parameters that were not significantly different in the LLLT group compared with the control group. The urine volume increase in non-diabetic rats after LLLT was an incidental observation that warrants future study.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Confidence Intervals
  • Diabetes Mellitus, Experimental / complications*
  • Disease Models, Animal
  • Immunohistochemistry
  • Lasers, Semiconductor / therapeutic use*
  • Low-Level Light Therapy / methods*
  • Male
  • Multivariate Analysis
  • Photomicrography
  • Radiation Dosage
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reference Values
  • Streptozocin
  • Wound Healing / physiology*
  • Wounds and Injuries / etiology
  • Wounds and Injuries / pathology
  • Wounds and Injuries / radiotherapy*


  • Streptozocin