Codon adaptation-based control of protein expression in C. elegans

Nat Methods. 2011 Mar;8(3):250-2. doi: 10.1038/nmeth.1565. Epub 2011 Jan 30.

Abstract

We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter.

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins / genetics*
  • Cell Division / genetics
  • Codon / genetics*
  • Gene Expression Regulation*
  • Genetic Techniques*
  • Protein Biosynthesis / genetics*

Substances

  • Caenorhabditis elegans Proteins
  • Codon
  • G protein regulator 1, C elegans
  • G protein regulator 2, C elegans