CE-MS in biomarker discovery, validation, and clinical application

Proteomics Clin Appl. 2011 Feb;5(1-2):9-23. doi: 10.1002/prca.201000058. Epub 2010 Nov 11.


CE coupled MS (CE-MS) has become an increasingly employed technology in proteome analysis with focus on the identification of biomarker peptides in clinical proteomics. In this review, we will cover technical aspects of CE-MS coupling and highlight the improvements made in the last few years. We examine CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. We discuss the principal theoretical and practical obstacles encountered when employing CE-MS (and most other proteomic technologies) for the analysis of body fluids for biomarker discovery. We will present several examples of a successful application of CE-MS for biomarker discovery, implications for disease diagnosis, prognosis, and therapy evaluation, and will discuss current challenges and possible future improvements.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aging / urine
  • Biomarkers / analysis*
  • Biomarkers / urine
  • Coronary Artery Disease / urine
  • Electrophoresis, Capillary / methods*
  • Humans
  • Kidney Diseases / urine
  • Mass Spectrometry / methods*
  • Proteomics* / methods


  • Biomarkers