Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes

Nature. 1990 Dec 6;348(6301):510-4. doi: 10.1038/348510a0.

Abstract

A complementary DNA encoding a voltage-gated chloride channel from Torpedo marmorata electric organ was cloned by expressing hybrid-depleted messenger RNA in Xenopus oocytes. The predicted protein has a sequence of 805 amino acids containing several putative membrane-spanning domains. Expression of the protein in Xenopus oocytes shows that it is sufficient for channel function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Membrane / ultrastructure
  • Chloride Channels
  • Chlorides*
  • Cloning, Molecular
  • DNA / genetics
  • Electric Conductivity
  • Gene Expression
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Membrane Proteins / ultrastructure
  • Molecular Sequence Data
  • Oocytes
  • RNA, Messenger / genetics
  • Solubility
  • Xenopus laevis

Substances

  • Chloride Channels
  • Chlorides
  • Membrane Proteins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/X56758