Generation of mouse ES cell lines engineered for the forced induction of transcription factors

Sci Rep. 2011;1:167. doi: 10.1038/srep00167. Epub 2011 Nov 23.

Abstract

Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Engineering / methods
  • Cell Line
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism*
  • Gene Expression Profiling
  • Genetic Engineering / methods
  • Mice
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics*

Substances

  • Recombinant Proteins
  • Transcription Factors