Abstract
In living color: efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual-color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / chemistry
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Actins / metabolism
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Cell Membrane / chemistry*
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Cell Membrane / metabolism
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Cytoskeleton / chemistry
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Cytoskeleton / metabolism
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Fluorescent Dyes / chemistry
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Green Fluorescent Proteins / chemistry*
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Green Fluorescent Proteins / metabolism
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HeLa Cells
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Humans
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Microscopy, Fluorescence / methods*
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Nanostructures / chemistry
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Transfection
Substances
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Actins
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Fluorescent Dyes
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enhanced green fluorescent protein
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Green Fluorescent Proteins