Wrinkle reduction in post-menopausal women consuming a novel oral supplement: a double-blind placebo-controlled randomized study

Int J Cosmet Sci. 2014 Feb;36(1):22-31. doi: 10.1111/ics.12087. Epub 2013 Sep 18.


Objective: The maintenance of youthful skin appearance is strongly desired by a large proportion of the world's population. The aim of the present study was therefore to evaluate the effect on skin wrinkling, of a combination of ingredients reported to influence key factors involved in skin ageing, namely inflammation, collagen synthesis and oxidative/UV stress. A supplemented drink was developed containing soy isoflavones, lycopene, vitamin C and vitamin E and given to post-menopausal women with a capsule containing fish oil.

Method: We have performed a double-blind randomized controlled human clinical study to assess whether this cocktail of dietary ingredients can significantly improve the appearance of facial wrinkles.

Results: We have shown that this unique combination of micronutrients can significantly reduce the depth of facial wrinkles and that this improvement is associated with increased deposition of new collagen fibres in the dermis.

Conclusion: This study demonstrates that consumption of a mixture of soy isoflavones, lycopene, vitamin C, vitamin E and fish oil is able to induce a clinically measureable improvement in the depth of facial wrinkles following long-term use. We have also shown, for the first time with an oral product, that the improvement is associated with increased deposition of new collagen fibres in the dermis.

Keywords: collagen synthesis; oral supplement; post-menopausal women; skin physiology; wrinkle reduction.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Aged
  • Ascorbic Acid / administration & dosage
  • Carotenoids / administration & dosage
  • Dietary Supplements*
  • Double-Blind Method
  • Female
  • Fish Oils / administration & dosage
  • Humans
  • Isoflavones / administration & dosage
  • Lycopene
  • Middle Aged
  • Patient Compliance
  • Placebos
  • Postmenopause*
  • Skin Aging / drug effects*
  • Vitamin E / administration & dosage


  • Fish Oils
  • Isoflavones
  • Placebos
  • Vitamin E
  • Carotenoids
  • Ascorbic Acid
  • Lycopene