Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV

Science. 1986 Mar 14;231(4743):1289-91. doi: 10.1126/science.2418504.


Approximately 80 percent of all human sera that react with antigens of HTLV-III, the etiologic agent of the acquired immune deficiency syndrome (AIDS), recognize protein bands at 66 and 51 kilodaltons. A mouse hybridoma was produced that was specific to these proteins. Repeated cloning of the hybridoma did not separate the two reactivities. The p66/p51 was purified from HTLV-III lysates by immunoaffinity chromatography and subjected to NH2-terminal Edman degradation. Single amino acid residues were obtained in 17 successive degradation cycles. The sequence determined was a perfect translation of the nucleotide sequence of a portion of the HTLV-III pol gene. The purified p66/51 had reverse transcriptase activity and the monoclonal immunoglobulin G specifically removed the enzyme activity from crude viral extract as well as purified enzyme.

MeSH terms

  • Acquired Immunodeficiency Syndrome / immunology
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Viral / genetics
  • Antigens, Viral / immunology
  • Antigens, Viral / isolation & purification
  • Base Sequence
  • Chromatography, Affinity
  • Deltaretrovirus / enzymology*
  • Deltaretrovirus / genetics
  • Deltaretrovirus / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Viral
  • Humans
  • Hybridomas / immunology
  • Mice
  • Mice, Inbred BALB C
  • RNA-Directed DNA Polymerase / genetics
  • RNA-Directed DNA Polymerase / immunology*
  • RNA-Directed DNA Polymerase / isolation & purification


  • Antibodies, Monoclonal
  • Antigens, Viral
  • RNA-Directed DNA Polymerase

Associated data

  • GENBANK/K03455