A human monoclonal antibody recognizing a surface antigen on stomach cancer cells

Jpn J Cancer Res. 1989 Jun;80(6):546-53. doi: 10.1111/j.1349-7006.1989.tb01674.x.


Lymph-node lymphocytes of a patient with stomach cancer were fused with the mouse-human heterohybridoma, HM-5. A clone (2F9) was isolated that showed stable production of an IgM antibody reactive with NUGC-4 stomach cancer cell line. This antibody reacted predominantly with a cell surface antigen on cell lines originating from gastro-intestinal cancer and adenocarcinoma of lung, whereas it was not generally reactive with other types of cancers, or with normal kidney cells or fibroblasts. Biotin-labeled 2F9 antibody clearly stained cell smears and the nude mouse tumor of NUGC-4, but it did not show a positive reaction with stomach cancer tissues obtained from more than 10 patients, indicating that the antigen detected is very weakly expressed on tumor cells or on a limited number of stomach cancers. The antigen shed from NUGC-4 cell line was detected in the culture supernatant. 2F9 antibody precipitated a glycoprotein with a molecular weight of over 200 kilodaltons as well as a possible glycolipid, from NUGC-4 cells labeled with [3H]glucosamine or [35S]-H2SO4. Periodic acid treatment of the tissue section decreased reactivity with 2F9 antibody, but heat, neuraminidase or protease treatment did not. These results suggested that the epitope is present on a carbohydrate moiety not containing sialic acid, and that a part of the antigen molecule is sulfated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Antigens, Neoplasm / analysis*
  • Antigens, Neoplasm / immunology
  • Antigens, Surface / analysis*
  • Antigens, Surface / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / analysis
  • Fetus / immunology
  • Fluorescent Antibody Technique
  • Humans
  • Hybridomas / immunology
  • Immunoenzyme Techniques
  • Immunoglobulin M / immunology
  • Immunosorbent Techniques
  • Lymphocytes / immunology
  • Membrane Glycoproteins / immunology
  • Mice
  • Mice, Nude
  • Molecular Weight
  • Neoplasm Transplantation
  • Periodic Acid / pharmacology
  • Stomach Neoplasms / immunology*
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Antigens, Surface
  • Epitopes
  • Immunoglobulin M
  • Membrane Glycoproteins
  • Periodic Acid