Molecular detection of nine clinically important non-O157 Escherichia coli serogroups from raw sheep meat in Chaharmahal-va-Bakhtiari province, Iran

Meat Sci. 2014 Aug;97(4):428-32. doi: 10.1016/j.meatsci.2014.02.007. Epub 2014 Feb 15.


STEC isolates and also stx-negative Escherichia coli isolates from sheep meat from the Chaharmahal-va-Bakhtiari province, Iran were analyzed for nine clinically important non-O157 serotypes by PCR. A total of 90 E. coli isolates were tested. Stx-positive and eae-positive E. coli isolates did not belong to the nine most clinically relevant non-O157 STECs. Of the 80 non-STEC isolates, two belonged to the O103 and two belonged to the O128 groups. Stx-negative E. coli O103 and O128 strains isolated have potential in acquiring stx genes and continuing into the digestive system of consumers. Further studies are needed to analyze virulence characteristics of these E. coli strains to determine their potential role in causing disease in humans. For the sake of public health, it is important to monitor and investigate non-O157 diarrheagenic E. coli strains in meat in order to control and prevent them.

Keywords: Escherichia coli; Meat; PCR; Sheep; Shiga toxin.

MeSH terms

  • Adhesins, Bacterial / genetics
  • Animals
  • Diet
  • Escherichia coli / genetics*
  • Escherichia coli O157 / genetics
  • Escherichia coli Proteins / genetics*
  • Food Microbiology*
  • Genes, Bacterial*
  • Humans
  • Iran
  • Meat / microbiology*
  • Polymerase Chain Reaction
  • Serogroup*
  • Serotyping
  • Sheep
  • Shiga Toxin / genetics*
  • Shiga-Toxigenic Escherichia coli / genetics


  • Adhesins, Bacterial
  • Escherichia coli Proteins
  • eaeA protein, E coli
  • Shiga Toxin