Susceptibility of EBV-carrying B cell lines to infection by HIV-1: variability of production of progeny virus and expression of viral antigens

Cancer Lett. 1989 Nov 30;48(2):95-103. doi: 10.1016/0304-3835(89)90043-8.


We have examined 3 different EBV-carrying B cell lines, in terms of ability to be super-infected by the human immunodeficiency virus (HIV-1), and have followed these lines, after infection by HIV-1, over a period of 3 months. We found significant variation among different HIV-1 strains in terms of the multiplicity of infection required to initiate infection in these EBV-positive cell lines. Persistent infection by HIV-1 in the absence of detectable cytopathic effects could be demonstrated, as evaluated by a variety of techniques, including reverse transcriptase assay and immunofluorescence. The results indicate also that all of these cell lines produced progeny HIV-1 intermittently, with large amounts of virus production on some days but not others. In contrast, they were all able to continuously express p24.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / microbiology*
  • Cell Transformation, Viral*
  • Cells, Cultured
  • HIV Antigens / analysis
  • HIV-1 / growth & development*
  • Herpesvirus 4, Human
  • Humans
  • RNA-Directed DNA Polymerase / metabolism
  • Virus Replication


  • HIV Antigens
  • RNA-Directed DNA Polymerase