Glucose increases interleukin-12 gene expression and production in stimulated peripheral blood mononuclear cells of type 2 diabetes patients

Biomed J. Sep-Oct 2014;37(5):293-7. doi: 10.4103/2319-4170.132887.

Abstract

Background: Lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of type 2 diabetes patients produce more interleukin (IL)-12 under glucose treatment. The aim of this study was to determine whether increased IL-12 response in hyperglycemic LPS-stimulated PBMCs is due to increased gene expression or osmolarity.

Methods: LPS-stimulated PBMCs of 13 type 2 diabetes patients and 8 healthy controls were used for culture in the presence or absence of glucose or mannitol for 24 h. The IL-12 gene expressions of PBMCs and IL-12 protein levels in supernatants were evaluated.

Results: After 24 h, the stimulated PBMCs of diabetes patients expressed more IL-12 mRNA and produced more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. Stimulated PBMCs of controls did not express more IL-12 mRNA and produce more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment.

Conclusions: Glucose increases the IL-12 production in stimulated PBMCs of diabetes patients through increased IL-12 gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Diabetes Mellitus, Type 2 / metabolism*
  • Female
  • Gene Expression Regulation* / drug effects
  • Glucose / metabolism*
  • Humans
  • Interleukin-12 / genetics*
  • Interleukin-12 / metabolism
  • Leukocytes, Mononuclear / metabolism*
  • Lipopolysaccharides / pharmacology
  • Male
  • Middle Aged

Substances

  • Lipopolysaccharides
  • Interleukin-12
  • Glucose