Generation of Cardiomyocytes from Pluripotent Stem Cells

Methods Mol Biol. 2016:1353:181-90. doi: 10.1007/7651_2014_173.

Abstract

The advent of pluripotent stem cells (PSCs) enabled a multitude of studies for modeling the development of diseases and testing pharmaceutical therapeutic potential in vitro. These PSCs have been differentiated to multiple cell types to demonstrate its pluripotent potential, including cardiomyocytes (CMs). However, the efficiency and efficacy of differentiation vary greatly between different cell lines and methods. Here, we describe two different methods for acquiring CMs from human pluripotent lines. One method involves the generation of embryoid bodies, which emulates the natural developmental process, while the other method chemically activates the canonical Wnt signaling pathway to induce a monolayer of cardiac differentiation.

Keywords: Cardiac differentiation; EB method; Human cardiomyocytes; Monolayer method; Pluripotent stem cells.

MeSH terms

  • Amides / pharmacology
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cellular Reprogramming*
  • Collagen / chemistry
  • Drug Combinations
  • Embryoid Bodies / cytology*
  • Embryoid Bodies / drug effects
  • Embryoid Bodies / metabolism
  • Enzyme Inhibitors / pharmacology*
  • Humans
  • Intercellular Signaling Peptides and Proteins / pharmacology
  • Laminin / chemistry
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / drug effects
  • Myocytes, Cardiac / metabolism
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / drug effects
  • Pluripotent Stem Cells / metabolism
  • Primary Cell Culture
  • Proteoglycans / chemistry
  • Pyridines / pharmacology
  • Pyrimidines / pharmacology
  • Wnt Signaling Pathway

Substances

  • Amides
  • Chir 99021
  • Drug Combinations
  • Enzyme Inhibitors
  • Intercellular Signaling Peptides and Proteins
  • Laminin
  • Proteoglycans
  • Pyridines
  • Pyrimidines
  • matrigel
  • Y 27632
  • Collagen