Aging. Lysosomal signaling molecules regulate longevity in Caenorhabditis elegans

Science. 2015 Jan 2;347(6217):83-6. doi: 10.1126/science.1258857.

Abstract

Lysosomes are crucial cellular organelles for human health that function in digestion and recycling of extracellular and intracellular macromolecules. We describe a signaling role for lysosomes that affects aging. In the worm Caenorhabditis elegans, the lysosomal acid lipase LIPL-4 triggered nuclear translocalization of a lysosomal lipid chaperone LBP-8, which promoted longevity by activating the nuclear hormone receptors NHR-49 and NHR-80. We used high-throughput metabolomic analysis to identify several lipids in which abundance was increased in worms constitutively overexpressing LIPL-4. Among them, oleoylethanolamide directly bound to LBP-8 and NHR-80 proteins, activated transcription of target genes of NHR-49 and NHR-80, and promoted longevity in C. elegans. These findings reveal a lysosome-to-nucleus signaling pathway that promotes longevity and suggest a function of lysosomes as signaling organelles in metazoans.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Animals
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Nucleus / metabolism
  • Lipase / metabolism
  • Lipid Metabolism
  • Longevity / genetics
  • Longevity / physiology*
  • Lysosomes / metabolism*
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Signal Transduction

Substances

  • Caenorhabditis elegans Proteins
  • LBP-8 protein, C elegans
  • Molecular Chaperones
  • NHR-49 protein, C elegans
  • NHR-80 protein, C elegans
  • Receptors, Cytoplasmic and Nuclear
  • LIPL-4 protein, C elegans
  • Lipase