Ancient and novel small RNA pathways compensate for the loss of piRNAs in multiple independent nematode lineages

PLoS Biol. 2015 Feb 10;13(2):e1002061. doi: 10.1371/journal.pbio.1002061. eCollection 2015 Feb.

Abstract

Small RNA pathways act at the front line of defence against transposable elements across the Eukaryota. In animals, Piwi interacting small RNAs (piRNAs) are a crucial arm of this defence. However, the evolutionary relationships among piRNAs and other small RNA pathways targeting transposable elements are poorly resolved. To address this question we sequenced small RNAs from multiple, diverse nematode species, producing the first phylum-wide analysis of how small RNA pathways evolve. Surprisingly, despite their prominence in Caenorhabditis elegans and closely related nematodes, piRNAs are absent in all other nematode lineages. We found that there are at least two evolutionarily distinct mechanisms that compensate for the absence of piRNAs, both involving RNA-dependent RNA polymerases (RdRPs). Whilst one pathway is unique to nematodes, the second involves Dicer-dependent RNA-directed DNA methylation, hitherto unknown in animals, and bears striking similarity to transposon-control mechanisms in fungi and plants. Our results highlight the rapid, context-dependent evolution of small RNA pathways and suggest piRNAs in animals may have replaced an ancient eukaryotic RNA-dependent RNA polymerase pathway to control transposable elements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / immunology
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism
  • DNA Methylation
  • DNA Transposable Elements / immunology
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / immunology
  • Drosophila melanogaster / metabolism
  • Gene Expression Regulation
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Data
  • Nematoda / classification
  • Nematoda / genetics*
  • Nematoda / immunology
  • Nematoda / metabolism
  • Phylogeny*
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism
  • RNA-Dependent RNA Polymerase / genetics
  • RNA-Dependent RNA Polymerase / metabolism
  • Ribonuclease III / genetics
  • Ribonuclease III / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • DNA Transposable Elements
  • MicroRNAs
  • RNA, Small Interfering
  • RNA-Dependent RNA Polymerase
  • Ribonuclease III

Associated data

  • GEO/GSE56651