Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue

Jason R Maher; Oranat Chuchuen; Marcus H Henderson; Sanghoon Kim; Matthew T Rinehart; Angela D M Kashuba; Adam Wax; David F Katz

doi:10.1364/BOE.6.002022

Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue

Biomed Opt Express. 2015 May 8;6(6):2022-35. doi: 10.1364/BOE.6.002022. eCollection 2015 Jun 1.

Authors

Jason R Maher¹, Oranat Chuchuen¹, Marcus H Henderson¹, Sanghoon Kim¹, Matthew T Rinehart¹, Angela D M Kashuba², Adam Wax¹, David F Katz³

Affiliations

¹ Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
² University of North Carolina Eshelman School of Pharmacy and University of North Carolina Center for AIDS Research, University of North Carolina, Chapel Hill, NC, USA ; Department of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA.
³ Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA ; Department of Obstetrics and Gynecology, Duke University, Durham, NC 27708, USA.

Abstract

We report the development of a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) instrument (CRS-OCT) capable of measuring analytes in targeted biological tissues with sub-100-micron spatial resolution. The OCT subsystem was used to measure depth-resolved tissue morphology and guide the acquisition of chemically-specific Raman spectra. To demonstrate its utility, the instrument was used to accurately measure depth-resolved, physiologically-relevant concentrations of Tenofovir, a microbicide drug used to prevent the sexual transmission of HIV, in ex vivo tissue samples.

Keywords: (170.4500) Optical coherence tomography; (170.5660) Raman spectroscopy; (170.6935) Tissue characterization.

Abstract

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