[Continuous cell subline A4C2/9K and its application to the african swine fever virus study]

Vopr Virusol. 2015;60(2):43-7.
[Article in Russian]


A new continuous cell subline A4C2/9K highly sensitive to the african swine fever virus (ASFV) was prepared. All the tested ASFV strains isolated in the Russian Federation in 2008-2013 proliferated in this cell culture exhibiting hemadsorption and accumulated at a titer of up to 6.5 Ig HAU50/cm3. The cell culture A4C2/9K can be used for ASFV isolation or determination of its infectious activity and serotype identity. The culture versions of the ASFV strain Stavropol 01/08 at passages 24 and 33 in the cell culture A4C2/9K lost their pathogenicity for pigs.

Publication types

  • English Abstract

MeSH terms

  • African Swine Fever / immunology*
  • African Swine Fever Virus / growth & development*
  • African Swine Fever Virus / immunology*
  • African Swine Fever Virus / pathogenicity*
  • Animals
  • Cell Line / cytology
  • Cell Line / virology*
  • Russia
  • Swine