Intraspinal Transplantation of Autologous Neurogenically-Induced Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Paraplegic Dogs without Deep Pain Perception Secondary to Intervertebral Disk Disease

Turk Neurosurg. 2015;25(4):625-32. doi: 10.5137/1019-5149.JTN.14502-15.2.


Aim: To investigate the effects of neurogenically-induced autologous bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in paraplegic dogs without deep pain perception (DPP) secondary to intervertebral disk disease (IVDD).

Material and methods: Seven dogs which could not be improved neurologically with conventional treatment modalities were included in the study. All dogs were diagnosed by magnetic resonance imaging and surgically treated. Each dog received two times a suspension of autologous 5.0x106 NIBM-MSCs, which were positive to CNPase and MAP-2, as well as to GFAP and beta III tubulin into the spinal cord through the hemilaminectomy defect percutaneously, with a 21-day interval.

Results: Two months after cell transplantation, there were no changes except for 1 gait score improvement for 1 of the cases. At the 4th month, gait score had improved 1 score in 5 cases, and one score progress was recorded in proprioception and nociception in 1 case. In eight months-followed up 4 cases were evaluated by the same parameter; gait score had improved in 3 cases, and propriception improved in 2 cases, and nociception improved in 3 cases.

Conclusion: Our findings suggest that utility of autologous NIBM-MSCs for cases with poor prognosis after IVDD can be a promising approach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2',3'-Cyclic-Nucleotide Phosphodiesterases / analysis
  • 2',3'-Cyclic-Nucleotide Phosphodiesterases / metabolism
  • Animals
  • Bone Marrow Cells*
  • Dog Diseases / therapy*
  • Dogs
  • Glial Fibrillary Acidic Protein / metabolism
  • Intervertebral Disc Degeneration / complications
  • Intervertebral Disc Degeneration / therapy*
  • Intervertebral Disc Degeneration / veterinary*
  • Intervertebral Disc Displacement / complications
  • Intervertebral Disc Displacement / therapy*
  • Intervertebral Disc Displacement / veterinary*
  • Lameness, Animal / etiology
  • Lameness, Animal / therapy
  • Magnetic Resonance Imaging
  • Mesenchymal Stem Cell Transplantation / methods*
  • Mitogen-Activated Protein Kinase 1 / metabolism
  • Neural Stem Cells*
  • Neurologic Examination
  • Pain Perception*
  • Paraplegia / etiology
  • Paraplegia / therapy*
  • Paraplegia / veterinary*
  • Spinal Cord / surgery*
  • Transplantation, Autologous
  • Treatment Outcome
  • Tubulin / metabolism


  • Glial Fibrillary Acidic Protein
  • Tubulin
  • Mitogen-Activated Protein Kinase 1
  • 2',3'-Cyclic-Nucleotide Phosphodiesterases

Supplementary concepts

  • Intervertebral disc disease