Measurement of K vitamins in animal tissues by high-performance liquid chromatography with fluorimetric detection

J Chromatogr. 1989 Dec 29;497:131-7. doi: 10.1016/0378-4347(89)80012-x.

Abstract

A highly sensitive method for measuring endogenous phylloquinone and menaquinones in animal tissues was developed, based on high-performance liquid chromatography with coulometric reduction and fluorimetric detection, following extraction from tissue homogenate and purification on a Sep-Pak silica cartridge followed by thin-layer chromatography. The detection limits of phylloquinone, menaquinone-4, -6, -10 and -13 were 40, 40, 50, 70 and 80 pg/g in rat liver, respectively.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Spectrometry, Fluorescence
  • Tissue Distribution
  • Vitamin K / analysis*
  • Vitamin K / pharmacokinetics

Substances

  • Vitamin K