Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor

Paul Béland; Oleksiy Krupin; Pierre Berini

doi:10.1364/BOE.6.002908

Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor

Biomed Opt Express. 2015 Jul 16;6(8):2908-22. doi: 10.1364/BOE.6.002908. eCollection 2015 Aug 1.

Authors

Paul Béland¹, Oleksiy Krupin², Pierre Berini³

Affiliations

¹ Dept. of Biomedical Engineering, University of Ottawa, 161 Louis Pasteur, Ottawa, K1N 6N5, Canada.
² Dept. of Biological and Chemical Engineering, University of Ottawa, 161 Louis Pasteur, Ottawa, K1N 6N5, Canada.
³ School of Electrical Engineering and Computer Science, University of Ottawa, 800 King Edward Avenue, Ottawa, K1N 6N5, Canada ; Dept. of Physics, University of Ottawa, 150 Louis Pasteur, Ottawa, K1N 6N5, Canada ; Centre for Research in Photonics, University of Ottawa, 800 King Edward Avenue, Ottawa, K1N 6N5, Canada.

Abstract

Experimentation demonstrates long-range surface plasmon polariton waveguides as a useful biosensor to selectively detect gram negative or gram positive bacteria in human urine having a low concentration of constituents. The biosensor can detect bacteria at concentrations of 10(5) CFU/ml, the internationally recommended threshold for diagnostic of urinary tract infection. Using a negative control urine solution of bacterial concentration 1000☓ higher than the targeted bacteria, we obtain a ratio of 5.4 for the positive to negative signals.

Keywords: (130.3120) Integrated optics devices; (170.0170) Medical optics and biotechnology; (170.7230) Urology; (230.7390) Waveguides, planar; (240.6680) Surface plasmons; (250.5403) Plasmonics; (280.1415) Biological sensing and sensors.