Long non-coding RNA HOTAIR regulates cyclin J via inhibition of microRNA-205 expression in bladder cancer

Cell Death Dis. 2015 Oct 15;6(10):e1907. doi: 10.1038/cddis.2015.269.


The level of microRNA-205 (miR-205) is commonly deregulated in a number of cancers. Through the screening of the microRNA expression profile in bladder cancer tissue and cell lines, we found that expression of miR-205 was significantly suppressed. In addition, the levels of miR-205 expression had a negative correlation with the degree of bladder cancer malignancy. However, the biological functions of miR-205 remained unclear. In this study, we have demonstrated that miR-205 had a role in the inhibition of proliferation, migration and invasion of bladder cancer cells. Moreover, we have identified cyclin J (CCNJ) gene, which is involved in cell cycle regulation, as a novel target for miR-205. Furthermore, a long non-coding RNA HOTAIR (HOX transcript antisense RNA) was observed to participate in the silencing of miR-205 in bladder cancer cells by breaking the balance of histone modification between H3K4me3 (histone H3 at lysine 4 methylation) and H3K27me3 on miR-205 promoter. This study elucidates an important role that miR-205 had in the regulation of proliferation, migration and invasion of bladder cancer cells, suggesting a potential therapeutic target for combating bladder cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line, Tumor
  • Cell Movement
  • Cyclins / genetics
  • Cyclins / metabolism*
  • Gene Expression
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mice, SCID
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Neoplasm Invasiveness
  • Neoplasm Transplantation
  • RNA, Long Noncoding / physiology*
  • Urinary Bladder Neoplasms / genetics
  • Urinary Bladder Neoplasms / metabolism*
  • Urinary Bladder Neoplasms / pathology


  • 3' Untranslated Regions
  • CCNJ protein, human
  • Cyclins
  • HOTAIR long untranslated RNA, human
  • MIRN205 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding