miR-409-3p suppresses breast cancer cell growth and invasion by targeting Akt1

Biochem Biophys Res Commun. 2016 Jan 8;469(2):189-95. doi: 10.1016/j.bbrc.2015.11.099. Epub 2015 Nov 26.


Altered levels and functions of microRNAs (miRNAs) are correlated with carcinogenesis. While miR-409-3p has been shown to play important roles in several cancer types, its function in the context of breast cancer (BC) remains unknown. In this study, miR-409-3p was significantly downregulated in BC tissues and cell lines, compared with the corresponding control counterparts. Overexpression of miR-409-3p inhibited BC cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. Notably, miR-409-3p induced downregulation of Akt1 protein through binding to its 3' untranslated region (UTR). Conversely, restoring Akt1 expression rescued the suppressive effects of miR-409-3p. Our data collectively indicate that miR-409-3p functions as a tumor suppressor in BC through downregulating Akt1, supporting the targeting of the novel miR-409-3p/Akt1 axis as a potentially effective therapeutic approach for BC.

Keywords: Akt1; Breast cancer; Invasion; Proliferation; miR-409-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Cell Proliferation / genetics*
  • Cells, Cultured
  • Gene Targeting
  • Genes, Tumor Suppressor
  • Humans
  • Mice
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness
  • Proto-Oncogene Proteins c-akt / metabolism*


  • MIRN409 microRNA, human
  • MicroRNAs
  • AKT1 protein, human
  • Proto-Oncogene Proteins c-akt