Abstract
A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Ampicillin Resistance
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Bacterial Proteins / genetics*
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Cloning, Molecular
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Escherichia coli / genetics*
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Gene Expression Regulation
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Genes, Bacterial*
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Molecular Weight
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Mutation
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Phenotype
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Plasmids*
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Protein Biosynthesis
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Restriction Mapping
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Transcription, Genetic
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Transduction, Genetic