Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein

J Jorda; D J Leibly; M C Thompson; T O Yeates

doi:10.1039/c6cc00851h

Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein

Chem Commun (Camb). 2016 Apr 11;52(28):5041-4. doi: 10.1039/c6cc00851h. Epub 2016 Mar 18.

Authors

J Jorda¹, D J Leibly, M C Thompson, T O Yeates

Affiliation

¹ UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA. yeates@mbi.ucla.edu.

Abstract

We report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Proteins / chemistry*
Molecular Structure
Nanostructures / chemistry*
Nanotechnology
Protein Engineering
Salmonella typhimurium / chemistry*

Substances

Bacterial Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding