Immunological distinction between acetylcholine receptor and the alpha-bungarotoxin-binding component on sympathetic neurons

Proc Natl Acad Sci U S A. 1977 Oct;74(10):4689-92. doi: 10.1073/pnas.74.10.4689.


Clone PC12 of a rat sympathetic neuron cell line binds alpha-bungarotoxin and exhibits carbamoylcholine-stimulated uptake of sodium ions. Concentrations of alpha-bungarotoxin that saturate the alpha-bungarotoxin-binding site have no effect on agonist-stimulated sodium uptake. Conversely, antibodies against eel acetylcholine receptor block the agonist-induced sodium flux but fail to recognize the alpha-bungarotoxin-binding component. Detergent extracts of the PC12 clone inhibit the ability of antibody to eel acetylcholine receptor to recognize 125I-alpha-bungarotoxin-acetylcholine receptor complexes derived from muscle. These results distinguish between a ganglionic nicotinic acetylcholine receptor and an alpha-bungarotoxin-binding component on these cells, and provide evidence for antigenic similarities between muscle acetylcholine receptor and ganglionic acetylcholine receptor.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylcholine / metabolism*
  • Binding Sites
  • Bungarotoxins / metabolism*
  • Cell Line
  • Neurons / metabolism*
  • Receptors, Cholinergic / immunology*
  • Sodium / metabolism
  • Sympathetic Nervous System / metabolism*


  • Bungarotoxins
  • Receptors, Cholinergic
  • Sodium
  • Acetylcholine