A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes

J Immunol Methods. 1989 Jun 2;120(1):125-31. doi: 10.1016/0022-1759(89)90297-4.


A new simplified radioassay for measuring polymorphonuclear leukocyte (PMN) chemotaxis is proposed using 51Cr-labeled cells and a single-filter system. The technique offers all the advantages described for the double-filter radioassay and permits a reproducible measurement of random locomotion, chemokinesis and chemotaxis. Moreover the single-filter radioassay utilizes commercially available and disposable chambers gathered in a multichamber apparatus; this makes the method very easy to learn and rapid to perform.

MeSH terms

  • Cell Movement
  • Chemotaxis, Leukocyte* / drug effects
  • Chromium Radioisotopes
  • Filtration / instrumentation
  • Humans
  • In Vitro Techniques
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / physiology*
  • Time Factors


  • Chromium Radioisotopes
  • N-Formylmethionine Leucyl-Phenylalanine