Lethality of cytochalasin B and other compounds isolated from fungus Aspergillus sp. (Trichocomaceae) endophyte of Bauhinia guianensis (Fabaceae)

André de O Feitosa; Amanda Cristina S Dias; Gisele da C Ramos; Heriberto R Bitencourt; José Edson S Siqueira; Patrícia Santana B Marinho; Andersson Barison; Fernanda M M Ocampos; Andrey Moacir do R Marinho

doi:10.1016/j.ram.2016.04.002

Lethality of cytochalasin B and other compounds isolated from fungus Aspergillus sp. (Trichocomaceae) endophyte of Bauhinia guianensis (Fabaceae)

Rev Argent Microbiol. 2016 Jul-Sep;48(3):259-263. doi: 10.1016/j.ram.2016.04.002. Epub 2016 Aug 24.

Authors

André de O Feitosa¹, Amanda Cristina S Dias¹, Gisele da C Ramos¹, Heriberto R Bitencourt¹, José Edson S Siqueira¹, Patrícia Santana B Marinho¹, Andersson Barison², Fernanda M M Ocampos², Andrey Moacir do R Marinho³

Affiliations

¹ Universidade Federal do Pará, Instituto de Ciências Exatas e Naturais-Programa de Pós-graduação em Química, Belém, Pará, Brazil.
² Universidade Federal do Paraná, Departamento de Química-Programa de Pós-graduação em Química, Curitiba, Paraná, Brazil.
³ Universidade Federal do Pará, Instituto de Ciências Exatas e Naturais-Programa de Pós-graduação em Química, Belém, Pará, Brazil. Electronic address: andrey@ufpa.br.

PMID: 27567521
DOI: 10.1016/j.ram.2016.04.002

Abstract

Endophytic fungi are fungi that colonize internal tissues of plants; several biologically active compounds have been isolated from these fungi. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, this study aimed the isolation and structural identification of ergosterol (1), ergosterol peroxide (2), mevalonolactone (3), cytochalasin B (4) and cytochalasin H (5) from Aspergillus sp. EJC 04, an endophytic fungus from Bauhinia guianensis. The cytochalasin B (4) and the diacetate derivative of cytochalasin B (4a) showed high lethality in the brine shrimp assay. This is the first occurrence of cytochalasins in Amazonian endophytic fungi from B. guianensis.

Keywords: Artemia salina; Endophytic fungi; Hongos endófitos; Metabolitos secundarios; Secondary metabolites.

Publication types

Comparative Study

MeSH terms

Acetylation
Animals
Argentina
Artemia / drug effects*
Aspergillus / chemistry*
Aspergillus / isolation & purification
Cytochalasin B / chemistry
Cytochalasin B / isolation & purification
Cytochalasin B / toxicity*
Cytochalasins / chemistry
Cytochalasins / isolation & purification
Cytochalasins / toxicity*
Endophytes / chemistry*
Endophytes / isolation & purification
Ergosterol / analogs & derivatives*
Ergosterol / chemistry
Ergosterol / isolation & purification
Ergosterol / toxicity
Fabaceae / microbiology*
Lethal Dose 50
Mevalonic Acid / analogs & derivatives*
Mevalonic Acid / chemistry
Mevalonic Acid / isolation & purification
Mevalonic Acid / toxicity
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Spectrometry, Mass, Electrospray Ionization
Structure-Activity Relationship

Substances

Cytochalasins
Cytochalasin B
cytochalasin H
mevalonolactone
Mevalonic Acid
ergosterol-5,8-peroxide
Ergosterol