Characterization of a mouse mitogen-regulated protein/proliferin gene and its promoter: a member of the growth hormone/prolactin gene superfamily

Biochim Biophys Acta. 1989 Sep 21;1009(1):75-82. doi: 10.1016/0167-4781(89)90081-x.


A genomic clone containing the complete gene, designated mrp/plf3, of a member of the mitogen-regulated protein/proliferin (MRP/PLF) multi-gene family was isolated from a mouse genomic library constructed in lambda L47.1. The DNA sequences of the 5' flanking region, the exons, and the exon/intron boundaries have been determined. The size and organization of the five exons in the murine mrp/plf3 gene are very similar to those of members of the prolactin/growth hormone family, confirming that mrp/plf3 is a part of this superfamily. Removal of an upstream negative regulatory element revealed an active mrp/plf promoter that was responsive to TGF-alpha and contains known regulatory elements. Intracellular mrp/plf mRNA levels were increased by 17 beta-estradiol and reduced by dexamethasone.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA / genetics*
  • Exons
  • Glycoproteins / genetics*
  • Growth Hormone / genetics*
  • Intercellular Signaling Peptides and Proteins
  • Introns
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Nucleic Acid Hybridization
  • Prolactin / genetics*
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping


  • Glycoproteins
  • Intercellular Signaling Peptides and Proteins
  • Prl2c2 protein, mouse
  • RNA, Messenger
  • Prolactin
  • Growth Hormone
  • DNA

Associated data

  • GENBANK/X16009
  • GENBANK/X16010
  • GENBANK/X16011
  • GENBANK/X16012
  • GENBANK/X16013