Transcriptome analysis of inflammation-related gene expression in endothelial cells activated by complement MASP-1

Sci Rep. 2017 Sep 5;7(1):10462. doi: 10.1038/s41598-017-09058-8.


Mannan-binding lectin-associated serine protease 1 (MASP-1), the most abundant enzyme of the complement lectin pathway, is able to stimulate human umbilical vein endothelial cells (HUVECs) to alter the expression of several cytokines and adhesion molecules. This study has assessed to what extent MASP-1 is able to modify the transcriptional pattern of inflammation-related (IR) genes in HUVECs. We utilized Agilent microarray to analyse the effects of recombinant MASP-1 (rMASP-1) in HUVECs, on a set of 884 IR genes. Gene Set Enrichment Analysis showed an overall activation of inflammation-related genes in response to rMASP-1. rMASP-1 treatment up- and down-regulated 19 and 11 IR genes, respectively. Most of them were previously unidentified, such as genes of chemokines (CXCL1, CXCL2, CXCL3), inflammatory receptors (TLR2, BDKRB2) and other inflammatory factors (F3, LBP). Expression of IR genes changed early, during the first 2 hours of activation. Both p38-MAPK inhibitor and NFκB inhibitor efficiently suppressed the effect of rMASP-1. We delineated 12 transcriptional factors as possible regulators of rMASP-1-induced IR genes. Our microarray-based data are in line with the hypothesis that complement lectin pathway activation, generating active MASP-1, directly regulates inflammatory processes by shifting the phenotype of endothelial cells towards a more pro-inflammatory type.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers
  • Computational Biology / methods
  • Endothelial Cells / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Gene Regulatory Networks
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Inflammation / genetics*
  • Inflammation / immunology
  • Inflammation / metabolism*
  • Mannose-Binding Protein-Associated Serine Proteases / metabolism*
  • Signal Transduction
  • Transcriptome*


  • Biomarkers
  • MASP1 protein, human
  • Mannose-Binding Protein-Associated Serine Proteases