Prognostic value of serum biomarkers of cerebral injury in classifying neurological outcome after paediatric resuscitation

Resuscitation. 2018 Jan:122:113-120. doi: 10.1016/j.resuscitation.2017.09.012. Epub 2017 Sep 20.


Aim: To investigate if the serum biomarkers of cerebral injury, neuron-specific enolase and S100b protein, may classify unfavourable neurological outcome after paediatric cardiac arrest.

Methods: We performed a retrospective study of neuron-specific enolase and S100b measurements from 95 children treated in our paediatric cardiac intensive care unit after cardiac arrest. Neurological outcome at discharge was evaluated using the paediatric cerebral performance category scale, with unfavourable outcome defined as a change of >1 compared to pre-arrest status or death.

Results: Fifty-eight patients (61.1%) survived to discharge with 48 (50.5%) having a favourable neurological outcome. We observed significantly higher levels of both biomarkers in the unfavourable outcome group at designated time points (neuron-specific enolase at 24, 48, and 72h and S100b at 12, 24, and 48h after cardiac arrest, p<0.05). Receiver operating characteristic areas under the curve for neuron-specific enolase were 0.83, 0.80, and 0.73 at time points 24, 48, and 72h and 0.87, 0.81, and 0.82 for S100b at 12, 24, and 48h after cardiac arrest, respectively. Neuron-specific enolase measurement at 24h after cardiac arrest was an independent predictor of unfavourable outcome in a multivariable analysis.

Conclusions: Neuron-specific enolase and S100b classify unfavourable neurological outcome in this large paediatric cardiac arrest cohort. Further multi-institutional prospective studies to comprehensively evaluate the diagnostic accuracy of these biomarkers under various clinical conditions and to determine reliable cut-off values in children are warranted.

Keywords: Biomarkers of cerebral injury; Neurological outcome; Neuron-specific enolase; Paediatric cardiac arrest; S100b.

MeSH terms

  • Adolescent
  • Biomarkers / blood
  • Brain Injuries / blood*
  • Brain Injuries / diagnosis
  • Brain Injuries / etiology
  • Cardiopulmonary Resuscitation / adverse effects*
  • Child
  • Child, Preschool
  • Female
  • Heart Arrest / blood*
  • Heart Arrest / mortality
  • Heart Arrest / therapy
  • Humans
  • Infant
  • Infant, Newborn
  • Intensive Care Units, Pediatric
  • Logistic Models
  • Male
  • Phosphopyruvate Hydratase / blood*
  • Predictive Value of Tests
  • ROC Curve
  • Retrospective Studies
  • S100 Calcium Binding Protein beta Subunit / blood*


  • Biomarkers
  • S100 Calcium Binding Protein beta Subunit
  • Phosphopyruvate Hydratase