Quantitative Detection of Horse Contamination in Cooked Meat Products by ELISA

J AOAC Int. 2018 May 1;101(3):817-823. doi: 10.5740/jaoacint.17-0151. Epub 2017 Oct 20.


Concerns about the contamination of meat products with horse meat and new regulations for the declaration of meat adulterants have highlighted the need for a rapid test to detect horse meat adulteration. To address this need, Microbiologique, Inc., has developed a sandwich ELISA that can quantify the presence of horse meat down to 0.1% (w/w) in cooked pork, beef, chicken, goat, and lamb meats. This horse meat authentication ELISA has an analytical sensitivity of 0.000030 and 0.000046% (w/v) for cooked and autoclaved horse meat, respectively, and an analytical range of quantitation of 0.05-0.8% (w/v) in the absence of other meats. The assay is rapid and can be completed in 1 h and 10 min. Moreover, the assay is specific for cooked horse meat and does not demonstrate any cross-reactivity with xenogeneic cooked meat sources.

MeSH terms

  • Animals
  • Cattle
  • Chickens
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Goats
  • Horses / immunology
  • Limit of Detection
  • Meat Products / analysis*
  • Red Meat / analysis*
  • Reproducibility of Results
  • Sheep
  • Swine