Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols

J Immunol. 1987 Apr 15;138(8):2422-6.


We have produced a new rat IgG monoclonal antibody against the murine transferrin receptor (TR). This antibody (C2F2) exhibits a surprisingly selective pattern of inhibition of murine lymphocyte activation protocols. C2F2 inhibits the mixed lymphocyte reaction (MLR) and the generation of cytotoxic T cells. Interestingly, although interleukin 1 (IL 1)-dependent thymocyte co-stimulatory activity is strongly inhibited by C2F2, interleukin 2 (IL 2)-dependent thymocyte co-stimulation is only marginally reduced. IL 2-dependent growth of CTLL cells is also not inhibited by C2F2. These data suggest that IL 1-dependent helper T cell activation is very sensitive to C2F2-mediated inhibition. Studies with phytohemagglutinin, Concanavalin A, and lipopolysaccharide induced activation also indicate that the inhibitory effects of C2F2 are selective, and T cell activation may be more sensitive to inhibition than B cell activation. Although there is little published information about the functional effects of other rat anti-mouse TR antibodies, the available data suggest that the patterns of inhibition produced by anti-TR antibodies may be individually distinct. Anti-TR antibodies may constitute a new set of highly selective probes for the study of lymphocyte activation.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • B-Lymphocytes / immunology*
  • Cytotoxicity, Immunologic
  • Immunoglobulin G / immunology*
  • Interleukin-1 / pharmacology
  • Interleukin-2 / pharmacology
  • Lymphocyte Activation*
  • Lymphocyte Culture Test, Mixed
  • Rats
  • Rats, Inbred WF
  • Receptors, Transferrin / immunology
  • Receptors, Transferrin / physiology*
  • T-Lymphocytes / immunology*
  • Transferrin / metabolism


  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Interleukin-1
  • Interleukin-2
  • Receptors, Transferrin
  • Transferrin